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KMID : 0882420080750020186
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Korean Journal of Medicine
2008 Volume.75 No. 2 p.186 ~ p.193
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The mechanism of action of neuropeptide Y in rat colonic motility
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Nah Byung-Kyu
Park Seon-Mee
Youn Sei-Jin
Chae Hee-Bok
Jeon Won-Joong
Yu Il-Young
Ko Byeong-Seong
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Abstract
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Background: Neuropepetide Y (NPY) is involved in the regulation of several gut functions, but the neuronal
action of NPY has not been fully investigated. This study was designed to investigate the effect and mechanism of action
of NPY on motility in the proximal and distal rat colon.
Methods: Rat colon with an intact superior mesenteric artery was isolated. After a basal period, NPY was administered at concentrations of 14 pM, 70 pM, 140 pM, and 280 pM. Intraluminal pressures were monitored in the proximal and distal colon. The contractile response was expressed as a percent change of motility indices over the basal level. After a pre-infusion of atropine (AT), tetrodotoxin (TTX), propranolol, hexamethonium, and phentolamine, NPY was infused at a concentration of 140 pM, and pressures were monitored.
Results: NPY increased the colonic motility at concentrations of 14, 70, 140, and 280 pM in the proximal colon
(28.5±28.2%, 48.4±34.3%, 122.9±97.3%, 68.2± 28.1%, respectively) and in the distal colon (44.9±25.9%, 103.8±72.0%,
237.1±131.0%, 93.0±63.9%, respectively) in a dose-dependent manner. The enhancing effect of NPY (140 pM) on colonic
motility was significantly suppressed by pretreatment with atropine, propranolol, and TTX. However, the effect of NPY
was not inhibited by hexamethonium or phentolamine.
Conclusion: NPY increases colonic motility. The enhancing effect of NPY on colonic motility may require cholinergic input via muscarinic receptors or adrenergic input via beta-receptors. (Korean J Med 75:186-193, 2008)
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KEYWORD
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Neuropeptide Y, Colon, Motility
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